Organoid Workflow without Animal-Based ECM

Hero banner for TheWell Bioscience announcing a reproducible, xeno-free organoid workflow without animal-based ECM, with lab vials and equipment on the right.

Move beyond the variability of animal-based ECM for a more reproducible, biologically relevant organoid workflow.

Batch-to-batch variability and difficult handling associated with animal-derived matrices like Matrigel remain persistent challenges in organoid research, affecting reproducibility, translatability, and scalability.

At TheWell Bioscience, we’ve built a fully xeno-free, end-to-end ecosystem specifically designed to help researchers standardize and simplify organoid development without sacrificing performance.

Key products for organoid culture

VitroGel® Xeno-Free Hydrogel

VitroGel® ORGANOID (1-5) are ready-to-use, xeno-free (animal origin-free) hydrogels that support the growth of patient-derived organoids or organoids developed from pluripotent stem cells (PSCs), co-culture, and PDX model. Designed for a simple, fast, and easy workflow, it allows room temperature handling and operation without the need for an ice bucket.

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RocketCell™ Organoid Xeno-Free Essential-Core Medium

A universal, chemically defined foundation medium that supports multiple organoid types with a single formulation—reducing reagent costs and waste by minimizing the need for multiple specialized media purchases.

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CytoGrow™ Growth Factors

CytoGrow™ is TheWell Bioscience’s portfolio of premium-grade recombinant proteins that pair seamlessly with RocketCell™ Organoid Xeno-Free Essential Core Medium to enhance cellular activity and drive superior performance.

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VitroPrime™ 3D Culture and Imaging Plate

Designed to keep organoids stable, this plate prevents floating and rotation while a dedicated medium-exchange channel allows complete media changes without disturbing or losing samples.

Fixation, washing, and staining can all be performed in the same vessel — eliminating transfers and sample loss. The ultra-clear #1.5 cover glass bottom supports long-term tracking and high-resolution imaging throughout the entire workflow.

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BONUS: Rapid organoid harvesting in 15 minutes.
Recover from VitroGel® or Animal-based (Fast 2-min ECM dissociation)

The non-enzymatic VitroGel® Organoid Recovery Solution delivers fast, gentle, and high-yield organoid recovery while preserving viability and structural integrity — enabling the recovery of more healthy, intact organoids for reliable downstream assays every time.

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Bottle of VitroGel Organic Recovery Solution set against a background of circular cell-like structures seen under a microscope; scale 500 μm shown.