Mix the hydrogel and cell suspension by gently pipetting up and down approximately 10 times. Then, position the pipette tip (micro or serological) adjacent to the edge of the inner protected chamber, slightly angled away from the wall, and slowly dispense the mixture while moving the tip along the perimeter. This technique promotes even distribution and ensures the hydrogel flows fully beneath the chamber lip for optimal anchoring.
Note: Please refer to the VitroGelĀ® Ready-to-Use hydrogel or VitroGelĀ® High Concentration hydrogel protocols to learn more about how to mix the gels with the cell suspension.
